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Nucleic Acids Research, 1982, Vol. 10, No. 24 7959-7964
© 1982

CHEMISTRY

A photoinduced cleavage of DNA useful for determining T residues

András Simoncsits1,* and István Török2

1Institute of Genetics 2Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences H-6701 Szeged, P.O. Box 521, Hungary

Received October 26, 1982. Accepted November 17, 1982.

Irradiation of 5‘ -[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T>G cleavage offers an alternative method for determining T residues in chemical DNA sequencing

*Present adderss: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH ENGLAND


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