Nucleic Acids Research, 1982, Vol. 10, No. 24 7965-7976
© 1982
MOLECULAR BIOLOGY |
Transcriptional ‘enhancers’ from SV40 and polyoma virus show a cell type preference
Institut für Molekularbiologie II der Universitat Zürich Hönggerberg 8093 Zürich, Switzerland *Biochemistry Division, National Institute for Medical Research, Mill Hill London NW7 1AA, UK
Received October 18, 1982. Accepted November 30, 1982.
Transcriptional "enhancers" have been identified in both the monkey virus SV40 and in the mouse polyoma virus. Here we report that these enhancers show a cell type preference. This was done, (i) by assaying for T antigen expression and viral DNA replication of polyoma DNA which contains its own enhancer or the enhancer of SV40 virus, and (ii) by linking either of the two enhancer elements to the rabbit ß1-globin gene and measuring transient globin expression in mouse and primate cells. The results were consistent in all the assays: In mouse cells the polyoma enhancer is slightly more effective than the SV40 enhancer. However, in primate cells the SV40 enhancer induces a four to six fold higher level of gene expression than does the polyoma enhancer.
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