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Nucleic Acids Research, 1982, Vol. 10, No. 24 7977-7991
© 1982

MOLECULAR BIOLOGY

Chromatin structure of histone genes in sea urchin sperms and embryos

Giovanni Spinelli*, Ida Albanese*, Letizia Anello+, Mirella Ciaccio+ and Italia Di Liegro

+Istituto di Biologia dello Sviluppo del Consiglio Nazionale delle Ricerche, via Archirafi 22 90123 Palermo, Italy *Istituto di Anatomia Comparata, Universitá di Palermo via Archirafi 20

Received October 18, 1982. Accepted November 18, 1982.

The nucleosomal organization of active and repressed {alpha} subtype histone genes has been investigated by micrococcal nuclease digestion of P. lividus sperm, 32-64 cell embryo and mesenchyme blastula nuclei, followed by hybridization with 32 P-labeled specific DNA probes. In sperms, fully repressed histone genes are regularly folded in nucleosomes, and exhibit a greater resistance to micrococcal nuclease cleavage than bulk chromation. In contrast, both coding and spacer {alpha} subtype histone DNA sequences acquire an altered conformation in nuclei from early cleavage stage embryos, i.e., when these genes are maximally expressed. Switching off of the {alpha} subtype histone genes, in mesenchyme blastulae, restores the typical nucleosomal organization on this chromatin region. As probed by hybridization to D.melanogaster actin cDNA actin genes retain a regular nucleosomal structure in all the investigated stages.


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