Nucleic Acids Research, 1982, Vol. 10, No. 24 7993-8006
© 1982
MOLECULAR BIOLOGY |
A nonanucleotide sequence involved in promotion of ribosomal RNA synthesis and RNA priming of DNA replication on yeast mitochondria
Section for Molecular Biology, Laboratory of Biochemistry, University of Amsterdam Kruislaan 318, 1098 SM Amsterdam, The Netherlands *Departments of Medicine, Biochemistry, Biology and Pathology, University of Chicago Chicago, IL 60637, USA
Received October 11, 1982. Accepted November 16, 1982.
We have examined the initiation of transcription of the mitochondrial genes for ribosomal RNA (rRNA) in the yeast Kluyveromyces lactis and show that these are transcribed independently from individual promoters. The mature large rRNA contains a 5‘ di-or triphosphate end which can be labeled in vitro with [
32p]GTP using guanylyltransferase and this enabled us to determine the nucleotide sequence of its 5‘ terminus. For the small rRNA, a minor in vitro capped RNA species hybridizes in the region where – as judged from S1 nuclease protection experiments – the precursor of this RNA starts. We have determined the DNA sequence around the beginning of both rRNA genes and this reveals the existence of an indentical nonanucleotide sequence (5‘ –ATATAAGTS– 3’) just preceding the positions where the rRNAs start. This sequence is identical to the one preceding the rRNA genes in the mtDNA of the distantly related yeast Saccharomyces cerevisiae (Osinga, K.A. and Tabak, H.F.(1982) Nucl. Acids Res. 10,3617-3626) and supports out proposal that this sequence motif is part of a yeast mitochondrial promoter.
We have noticed that the same sequence is located in the putative origin of replication present in hypersuppressive petite mutants of S. Cerevisiae and consider the possibility that this sequence is involved in RNA priming of DNA replication.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Gomez-Gonzalez and A. Aguilera R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX Nucleic Acids Res., July 1, 2009; 37(13): 4315 - 4321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-Q. Tang, S. Paratkar, and S. S. Patel Fluorescence Mapping of the Open Complex of Yeast Mitochondrial RNA Polymerase J. Biol. Chem., February 27, 2009; 284(9): 5514 - 5522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. V. Mirkin and S. M. Mirkin Replication Fork Stalling at Natural Impediments Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 13 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Rogowska, O. Puchta, A. M. Czarnecka, A. Kaniak, P. P. Stepien, and P. Golik Balance between Transcription and RNA Degradation Is Vital for Saccharomyces cerevisiae Mitochondria: Reduced Transcription Rescues the Phenotype of Deficient RNA Degradation Mol. Biol. Cell, March 1, 2006; 17(3): 1184 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Matsunaga and J. A. Jaehning A Mutation in the Yeast Mitochondrial Core RNA Polymerase, Rpo41, Confers Defects in Both Specificity Factor Interaction and Promoter Utilization J. Biol. Chem., January 16, 2004; 279(3): 2012 - 2019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Bullerwell, L. Forget, and B. F. Lang Evolution of monoblepharidalean fungi based on complete mitochondrial genome sequences Nucleic Acids Res., March 15, 2003; 31(6): 1614 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Karlok, S.-H. Jang, and J. A. Jaehning Mutations in the Yeast Mitochondrial RNA Polymerase Specificity Factor, Mtf1, Verify an Essential Role in Promoter Utilization J. Biol. Chem., July 26, 2002; 277(31): 28143 - 28149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Lisowsky, D. Wilkens, T. Stein, B. Hedtke, T. Borner, and A. Weihe The C-terminal Region of Mitochondrial Single-subunit RNA Polymerases Contains Species-specific Determinants for Maintenance of Intact Mitochondrial Genomes Mol. Biol. Cell, July 1, 2002; 13(7): 2245 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Costanzo, N. Bonnefoy, E. H. Williams, G. D. Clark-Walker, and T. D. Fox Highly Diverged Homologs of Saccharomyces cerevisiae Mitochondrial mRNA-Specific Translational Activators Have Orthologous Functions in Other Budding Yeasts Genetics, March 1, 2000; 154(3): 999 - 1012. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. F. Cliften, J.-Y. Park, B. P. Davis, S.-H. Jang, and J. A. Jaehning Identification of three regions essential for interaction between a sigma -like factor and core RNA polymerase Genes & Dev., November 1, 1997; 11(21): 2897 - 2909. [Abstract] [Full Text] [PDF]
|
|