Skip Navigation

This Article
Right arrow Print PDF (3173K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (64)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Koski, R. A.
Right arrow Articles by Hall, B. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koski, R. A.
Right arrow Articles by Hall, B. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 24 8127-8143
© 1982

Articles

An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription

Raymond A. Koski*, Darniel s. Allison, Marlis Worthington and Benjamin D. Hall

Department of Genetics, SK-50, University of Washington Seattle, WA 98195, USA

Received September 2, 1982. Accepted November 19, 1982.

A soluble cell–free extract containing RNA polymerase III – and factors essential for selective transcription of the yeast SUP4–tRNAtyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA Polymerase III was identified within the yeast tRNAtyr coding sequence by testing several SUP4 genes with 5'– and 3'–terminal deletions in the homologous transcription system.

Thirty–four different SUP4 genes with spontaneous mutations were also tested in the invitro system. Two point mutations drastically reduced transcription initiation two other mutations caused premature termination. These mutations have nearly identical effects on SUP4 gene transcription by Xenopus RNA polymerase III (I), which demonstrates that the essential features of RNA polymerase III transcription initiation and termination signals have been conserved throughout the course of eukaryotic evolution.

*Current address: Applied Molecular, Inc., 1892 Oak Terrace Lane, Newbury Park, CA 91320, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. Guffanti, R. Ferrari, M. Preti, M. Forloni, O. Harismendy, O. Lefebvre, and G. Dieci
A Minimal Promoter for TFIIIC-dependent in Vitro Transcription of snoRNA and tRNA Genes by RNA Polymerase III
J. Biol. Chem., August 18, 2006; 281(33): 23945 - 23957.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Ouyang, M. J. Martinez, L. S. Young, and K. U. Sprague
TATA-Binding Protein-TATA Interaction Is a Key Determinant of Differential Transcription of Silkworm Constitutive and Silk Gland-Specific tRNAAla Genes
Mol. Cell. Biol., February 15, 2000; 20(4): 1329 - 1343.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.