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Nucleic Acids Research, 1982, Vol. 10, No. 24 8155-8170
© 1982

Articles

Localization of human variable and constant region immunoglobulin heavy chain genes on subtelomeric band q32 of chromosome 14

O. Wesley McBride1, James Battey3, Gregory F. Hollis3, David C. Swan2, Ulrich Siebenlist3 and Philip Leder3

1Laboratories of Biochemistry 2Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health Bethesda, MD 20205 3Department of Genetics, Harvard Medical School Boston, MA 02115, USA

Received August 18, 1982. Accepted November 15, 1982.

Analysis of a group of human/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human variable region (VH), junctional (JH), and constant region (C{varepsilon}) heavy chain immunoglobulin genes indicates that all of these IgH genes are localized on the subtelomeric (q32) band of chromosome 14. Somatic cell hybrids were isolated in selective medium after fusing human fibroblasts with hprt– Chinese hamster cells. The human parental cells contained two translocation chromosomes representing a reciprocal translocation between chromosomes X and 14. Only those hybrid cell lines retaining a complete human autosome 14 or the X/14 translocation chromosome (i.e. containing band 14q32) retained the human IgH genes. Retention of these genes did not correlate with the presence of the other translocation chromosome, 14/X. These results indicate that all human IgH genes (VH, JH, and CH)map to the same chromosomal band (14q32) which is commonly involved in reciprocal translocations with human chromosome 8 (8q24) in B–cell neoplasms.


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