Skip Navigation

This Article
Right arrow Print PDF (515K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (52)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Winkle, S. A.
Right arrow Articles by Krugh, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Winkle, S. A.
Right arrow Articles by Krugh, T. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 24 8211-8223
© 1982

CHEMISTRY

On the cooperative and noncooperative binding of ethidium to DNA

Stephen A. Winkle{ddagger}, Leonard S. Rosenberg and Thomas R. Krugh*

Department of Chemistry, University of Rochester Rochester, NY 14627, USA

*To whom correspondence and reprint requests should be addressed.

Received July 14, 1982. Revised September 9, 1982. Accepted September 28, 1982.

The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dc).poly(dG-dC) has been studied by both phase partition and direct spectrophometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E.coli DNA. on the other hand, no evidence of coperative binding was observed in the binding isotherms obtained with calf thymus,C. perfringens, M.Lysodeikticus, or poly(dG-dC).(dG-dC) under the experimental conditions used (0.1 M Nacl).

{ddagger}Present address: Department of Chemistry, Busch Campus, Rutgers University, The State University, The State University of New Jersey, New Brunswick, New Jersey 08903


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.