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Nucleic Acids Research, 1982, Vol. 10, No. 3 1105-1112
© 1982

MOLECULAR BIOLOGY

Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence

Richard Herrmann

Mikrobiologie, Universität Heidelberg Im Neuenheimer Feld 230, 6900 Heidelberg, FRG

Received October 19, 1981. Revised December 15, 1981. Accepted December 15, 1981.

A 693 basepair cloned fragment of bacteriophage T4 DNA, which supports specifically growth of T4 amber mutants in gene 57, has been sequenced. A polypeptide can be deduced from this sequence, that is either 54 or 60 amino acids long depending which of two AUG codons, 18 nucleotides apart, are used for initiation. The size of this deduced polypeptide is compatible with the size of a single polypeptide (based on polyacrylamide gel electrophoresis) synthesized in vivo in E. coli under the direction of the cloned T4 DNA fragment.


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