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Nucleic Acids Research, 1982, Vol. 10, No. 3 967-977
© 1982

MOLECULAR BIOLOGY

Inducer-responsive expression of the cloned human interferon ß1 gene introduced into cultured mouse cells

Shigeo Ohno and Tadatsugu Taniguchi

Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research Tokyo 170, Japan

Received October 20, 1981. Revised December 21, 1981. Accepted December 21, 1981.

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN- ß1 ) gene and its flanking sequences. Cultured mouse FH3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-ß1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C) . In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-ß1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-ß1 gene.


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