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Nucleic Acids Research, 1982, Vol. 10, No. 3 979-991
© 1982

MOLECULAR BIOLOGY

DNA sequence of an Immediate-early gene (IE mRNA-5) of herpes simplex virus type I

Roger J. Watson and George F.Vande Woude

Laboratory of Molecular Oncology, National Cancer Institute, National Institutes of Health Bethesda, MD 20205, USA

Received October 13, 1981. Revised December 1, 1981. Accepted December 1, 1981.

We describe a 2560 base pair herpes simplex virus type 1 (HSV-1) DNA sequence containing the entire immediate-early mRNA-5 (IEmRNA-5) gene. The 3' and 5' termini of IEmRNA-5 were mapped within this DNA sequence by single-strand specific endonuclease protection experiments. The IEmRNA-5 gene contains DNA sequences from both the unique (Us) and reiterated (TRs/IRs) regions of the HSV-1 DNA short component and is interrupted by a single intron mapping in TRs/IRs. A search of the transcribed DNA sequence revealed no initiator codon within TRs/IRs. The first ATG was located 6 bases into Us sequences and this reading frame was open for a further 87 codons. A second longer open reading frame (316 codons) was also observed in the 3' transcribed region. The oligonucleotide sequences adjacent to the IEmRNA-5 termini are discussed in relation to those of the HSV-1 thymidine kinase gene and other genes transcribed by RNA polymerase II.


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