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Nucleic Acids Research, 1982, Vol. 10, No. 5 1503-1513
© 1982

MOLECULAR BIOLOGY

The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila

Nanni Din*,, Jan Engberg+ and Joseph G. Gall*

*Department of Biology, Yale University New Haven, CT 06511, USA +Department of Biochemistry B, Panum Institute, University of Copenhagen Copenhagen, Denmark

Received December 23, 1981. Accepted February 5, 1982.

The sequence of 415 nucleotides surrounding the transcription termination site for ribosomal RNA in Tetrahymena thermophila has been determined. The positions of the 3'-ends of mature 26S rRNA, pre-26S rRNA and 35S pre-rRNA were localized within this sequence by hybridization of the purified RNA species to selected DMA fragments, followed by S1 nuclease treatment of the hybrid and a precise sizing of the RNA-protected DNA fragments on sequencing gels. The 35S pre-rRNA population contained molecules with two distinct 3'-ends, one of which is identical to the end of pre-26S and 26S rRNA, while the other corresponds to a position 15 nucleotides further downstream, which is assumed to be the transcription termination site. The non-coding DNA strand contains a cluster of T's at the putative termination site, and several other T clusters are found further downstream. A short inverted repeat sequence is located near the putative termination site within the transcribed region. The possible role of these structures for transcription termination is discussed.

Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark


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