Nucleic Acids Research, 1982, Vol. 10, No. 5 1635-1652
© 1982
MOLECULAR BIOLOGY |
RNA polymerase-dependent mechanism for the stepwise T7 phage DNA transport from the virion into E. coli
M.M.Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences Moscow, USSR
Received November 17, 1981. Revised February 2, 1982. Accepted February 2, 1982.
The influence of rifampicin, streptolydigin, tetracycline, and chloramphenicol on phage DNA transport from the T7 virion into the E. coli cell was studied. It has been found that the DNA transport proceeds in at least three stages. During the initial stage the phage injects into the host cell the left 10 per cent of its DNA molecule. The entrance of the next 50 per cent of T7 DNA molecule is blocked by inhibitors which block transcription but not translation. Moreover, the entrance time of this part of the T7 DNA increases in the case of the T7 mutant D11 (which contains a deletion of the A2 and A3 promoters) and decreases in the case of the D53 mutant (which containsa deletion in the region of the early gene transcription terminator). It would appear, that the second stage of the phage DNA transport is tightly coupled with its transcription and that a mechanical function is carried out by RNA polymerase. The translation inhibitors completely block the entrance of the remaining 40 per cent of the T7 DNA molecule (class III genes) into the host cell. It would appear that some class I and (or) II gene product(s) are obligatory components of the final stage of T7 DNA transport. Some probable consequences of this virus DNA transport model as well as its agreement with the functional structure of T7 chromosome and with T7 development are discussed.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Kruse, B. Blagoev, A. Lobner-Olesen, M. Wachi, K. Sasaki, N. Iwai, M. Mann, and K. Gerdes Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli Genes & Dev., January 1, 2006; 20(1): 113 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Kivela, R. Daugelavicius, R. H. Hankkio, J. K. H. Bamford, and D. H. Bamford Penetration of Membrane-Containing Double-Stranded-DNA Bacteriophage PM2 into Pseudoalteromonas Hosts J. Bacteriol., August 15, 2004; 186(16): 5342 - 5354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dworkin and R. Losick Does RNA polymerase help drive chromosome segregation in bacteria? PNAS, October 29, 2002; 99(22): 14089 - 14094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grigoriev A relationship between gene expression and protein interactions on the proteome scale: analysis of the bacteriophage T7 and the yeast Saccharomyces cerevisiae Nucleic Acids Res., September 1, 2001; 29(17): 3513 - 3519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Garcia and I. J. Molineux Translocation and specific cleavage of bacteriophage T7 DNA in vivo by EcoKI PNAS, October 26, 1999; 96(22): 12430 - 12435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.W. Studier and J.J. Dunn Organization and Expression of Bacteriophage T7 DNA Cold Spring Harb Symp Quant Biol, January 1, 1983; 47(0): 999 - 1007. [Abstract] [PDF]
|
|
|
|
|
|
D. Endy, L. You, J. Yin, and I. J. Molineux Computation, prediction, and experimental tests of fitness for bacteriophage T7 mutants with permuted genomes PNAS, May 9, 2000; 97(10): 5375 - 5380. [Abstract] [Full Text] [PDF]
|
|