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Nucleic Acids Research, 1982, Vol. 10, No. 5 1661-1678
© 1982

MOLECULAR BIOLOGY

Separation and sequence of the 3' termini of M double-stranded RNA from killer yeast

Dennis J. Thiele, Regina W. Wang and Michael J. Leibowitz

Department of Microbiology, College of Medicine and Dentistry of New Jersey, Rutgers Medical School Piscataway, NJ 08854, USA

Received November 16, 1981. Revised February 1, 1982. Accepted February 9, 1982.

Four subspecies of M double-stranded RNA from a killer strain of Saccharomyces cerevisiae were isolated. Each subspecies was susceptible to heat cleavage, presumably at an internal 190 base pair A,U-rich region, generating two discrete fragments corresponding to each side of the A, U-rich region. Enzymatic and chemical RNA sequence analysis defined the 3'-terminal 175 bases for the larger fragment (M-1) and 231 bases for the smaller fragment (M-2). All four subspecies of M have identical size and 3'-terminal sequences. Potential translation initiation codons are present on the corresponding 5' termini of both fragments, and a possible 18S ribosomal RHA binding site is also present on the 5' terminus of M-1. Stem and loop structures for the 5' and 3' termini of M-1 may function as recognition sites for replication, transcription, and translation.


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