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Nucleic Acids Research, 1982, Vol. 10, No. 5 1679-1689
© 1982

MOLECULAR BIOLOGY

T-DNA of pTi-15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor

Norimoto Murai and John D. Kemp

Department of Plant Pathology, University of Wisconsin, and Plant Disease Resistance Research Unit ARS, USDA, Madison, WI 53706, USA

Received November 6, 1981. Revised January 27, 1982. Accepted January 27, 1982.

Northern blot hybridization analysis of polysomal polyadenylated RNA isolated from sunflower crown gall tumor PSCG-15955 demonstrated that a minimum of seven RNAs were transcribed from T-DNA of pT1-l5955 from Agrobacterium tumefaciens. The sizes of the T-DNA transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. The relative abundance of these polyadenylated RNAs varied greatly, the 1.0 kb RNA being the most abundant and the 1.6 kb RNA being the least abundant. Assignment of map locations of the seven polyadenylated RNAs indicated that the conserved region of T-DNA which may play a central role in tumorigenesis contained four RNAs of 1.8, 1.1, 0.8(a) and a portion of 0.8(b) kb long.


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N. Murai, J. D. Kemp, D. W. Sutton, M. G. Murray, J. L. Slightom, D. J. Merlo, N. A. Reichert, C. Sengupta-Gopalan, C. A. Stock, R. F. Barker, et al.
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Science, November 4, 1983; 222(4623): 476 - 482.
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