Nucleic Acids Research, 1982, Vol. 10, No. 5 1721-1732
© 1982
CHEMISTRY |
Differential stabilization by netropsin of inducible B-like conformations in deoxyribo-, ribo- and 2'-deoxy-2'-fluororibo-adenosine containing duplexes of (dA)n(dT)n and (dA)n(dU)na
Service de Biochimie, Bat. 142, Département de Biologie, Centre d'Etudes Nucléaires de Saclay F-91191 Gif-sur-Yvette, Cedex, France
Received January 19, 1982. Revised February 15, 1982. Accepted February 15, 1982.
Six polynucleotide duplexes containing polydeoxyadenylic acid, polyadenylic acid or poly-2'-deoxy-2'-fluoro-adenylic acid in one strand, and polydeoxyuridylic acid or polydeoxythymidylic acid in the other strand have been studied by circular dichroism, ionic strength dependence of melting temperatures and binding of the DNA specific antibiotic netropsin. Ciroular dichroisa spectra of (dA)n(dT)n and (dA)n(dU)n indicated the presence of the B-form of DNA, while those of (dAf1)n(dT)n and (rA)n(dT)n (and the corresponding (dU)n hybrids) indicated the presence of the A-form. (dAf1)n(dT)n and (dAf1)n(dU)n bound netropsin only slightly less than the (dA)n containing duplexes, while replacement by (rA)n decreased netropsin binding to a large degree. Since netropsin requires B-DNA for binding, it is concluded that the A to B transition is facilitated in the case of fluorine substitution in the sugar moiety, while the 2'-OH group greatly limits this conformational change.
aPart 3 in the series "Polynueleotide helix geometry and stability"; part 2 is ref. 14. Unusual abbreviations: (dAf)n: poly-2'-deoxy-2'-fluoro-adenylic acid; CD: circular dichroism.
bPresent Address: Forschungszentrum für Molekularbiologie und experimentelle Therapie, Akademie der Wissenschaften der DDR, DDR-69-JEHA, G.D.R.
cPresent Address: Division of Biophysios, The Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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