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Nucleic Acids Research, 1982, Vol. 10, No. 5 1733-1740
© 1982

MOLECULAR BIOLOGY

Replication of {lambda} dv DNA in vitro

Alfons Anderl and Albrecht Klein

Institute of Microbiology, University of Heidelberg Im Neuenheimer Feld 230, 6900 Heidelberg 1, FRG

Received January 14, 1982. Accepted February 11, 1982.

Exogenous {lambda} dv DNA was replicated in extracts prepared from E. coli cells carrying plasmids with inducible {lambda} 0 and/or P genes. Extracts from cells carrying only one of the two {lambda} replication functions complement each other in this reaction. The reaction further requires ribonucleotide triphosphates, an ATP regenerating system, DNA gyrase and RNA polymerase functions. Density labelling of the superhelical reaction products results in hybrid density indicating that one complete round of replication has taken place in vitro.


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[Abstract] [PDF]


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