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Nucleic Acids Research, 1982, Vol. 10, No. 5 1755-1769
© 1982

CHEMISTRY

Solid phase synthesis of polynucleotides. VI. Farther studies on polystyrene copolymers for the solid support

Hirataka Ito, Yoshimasa Ike, Satoshi Ikuta and Keiichi Itakura

Department of Molecular Genetics, City of Hope Research Institute 1450 East Duarte Road, Duarte, CA 91010, USA

Received January 5, 1982. Accepted February 1, 1982.

A simple solid phase method for the synthesis of oligodeoxyribonucleotides has been developed using the phosphotriester approach. Mononucleotide coupling units are sequentially added to the polystyrene copolymer with 1% divinylbenzene and two kinds of oligonucleotides, d(CACGACCCCTCCACGT) and d(AACTGGTATTACTGGGCG), are synthesized in a relatively high yield. One cycle of the mononucleotide addition is about 70 minutes, and this method is particularly suitable for the automation of the synthesis upon availability of an automatic synthesizer.


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