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Nucleic Acids Research, 1982, Vol. 10, No. 5 1799-1810
© 1982

MOLECULAR BIOLOGY

Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids

Brian Seed

Division of Biology, California Institute of Technology Pasadena, CA 91125, USA

Received November 10, 1981. Revised February 5, 1982. Accepted February 5, 1982.

We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.


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