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Nucleic Acids Research, 1982, Vol. 10, No. 6 1867-1876
© 1982

MOLECULAR BIOLOGY

A comparison of experimental and theoretical melting maps for replicative form of {varphi}X174 DNA

Yu.L. Lyubchenko, Yu.A. Kalambet, V.I. Lyamichev and A.S. Borovik

Institute of Molecular Genetics, USSR Academy of Sciences Kurchatov Sq. 46, Moscow 123182, USSR

Received January 19, 1982. Accepted February 23, 1982.

A previously elaborated technique for fixing a chosen partially melted state of DHA with glyoxal was used in a study of the melting process of the replicative form (RF III) of {varphi}X174DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoretical melting maps obtained in (4) and (6). Ibis comparison clearly shows that only rigorous calculations (4) and not the ones proposed by Azbel (6,7) correctly predict the course of RP III melting.


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L. Shokri, M. J. McCauley, I. Rouzina, and M. C. Williams
DNA Overstretching in the Presence of Glyoxal: Structural Evidence of Force-Induced DNA Melting
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[Abstract] [Full Text] [PDF]


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