Skip Navigation

This Article
Right arrow Print PDF (1408K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hinton, D. M.
Right arrow Articles by Gumport, R. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hinton, D. M.
Right arrow Articles by Gumport, R. I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 6 1877-1894
© 1982

MOLECULAR BIOLOGY

The preparative synthesis of oligodeoxyribonucleotides using RNA ligase

Deborah M. Hinton1, Catherine A. Brennan and Richard I. Gumport

Department of Biochemistry, School of Basic Medical Sciences and School of Chemical Sciences, University of Illinois Urbana, IL 61801, USA

Received January 18, 1982. Accepted February 17, 1982.

The synthesis of nmol quantities of defined sequences of oligodeoxyribonucleotides using T4 RNA ligase has been demonstrated. Reactions using from 18 to 200 nmol of substrates in which a single 2'-deoxyribonucleoside 3',5'-bisphosphate was added to an oligodeoxyribonucleotide resulted in yields from 13 to 95%. When two oligodeoxyribonucleotides were similarly joined using RNA ligase, the yields ranged from 10 to 50%. Although the reactions contained high concentrations of enzyme and were incubated from 5 to 21 days, there was little degradation of either substrates or products. We have also characterized an unusual product which arises when 3'-phosphate terminated oligodeoxyribonucleotides are incubated with RNA ligase and high concentrations of ATP. This product has an adenylyl group linked to the 3'-phosphate by an anhydride bond. The mechanistic and synthetic implications of forming this product are discussed.

1Present address: Cancer Biology Program, NCI -Frederick Cancer Research Facility, P.O. Box B, Frederick, MD, 21701, USA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. Billy, D. Hess, J. Hofsteenge, and W. Filipowicz
Characterization of the Adenylation Site in the RNA 3'-Terminal Phosphate Cyclase from Escherichia coli
J. Biol. Chem., December 3, 1999; 274(49): 34955 - 34960.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.