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Nucleic Acids Research, 1982, Vol. 10, No. 6 1963-1979
© 1982

MOLECULAR BIOLOGY

Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse

Ryo Kominami, Yukio Mishima, Yoshio Urano, Masaharu Sakai and Masami Muramatsu

Department of Biochemistry, Cancer Institute, 1-37-1, Kami-Ikebukuro, Toshima-ku Tokyo 170, Japan

Received January 5, 1982. Revised March 1, 1982. Accepted March 1, 1982.

An Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a {lambda}-phage vector. The site of transcription termination and the processed 3'-end of 28S RNA were determined on the cloned fragment and the surrounding nucleotide sequence determined.

The 3'-terminal nucleotides of mouse 28S RNA are similar to those of yeast, Drosophila and Xenopus although the homology was lost drastically beyond the 3'-end of 28S RNA. 45S precursor RNA terminated at 30 nucleotides downstream from the 3'-end of 28S RHA gene. A structure of a dyad symmetry with a loop was found immediately prior to the termination site of 45S RNA. The rDNA termination site thus shares some common features with termination sites recognized by other RNA polymerases.


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