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Nucleic Acids Research, 1982, Vol. 10, No. 6 1981-1994
© 1982

MOLECULAR BIOLOGY

Isolation and characterization of a human collagen {alpha} 1(I)-like gene from a cosmid library

Elisabeth H. Weiss, Kathryn S.E. Cheah*, Frank G Grosveld, Hans Henrik M. Dahl+, Ellen Solomon* and Richard A. Flavell

Laboratory of Gene Structure and Expression, National Institute for Medical Research Mill Hill, London NW7 1AA *Laboratory of Somatic Cell Genetics, Imperial Cancer Research Fund Laboratories P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK

Received January 4, 1982. Revised March 5, 1982. Accepted March 5, 1982.

We have isolated a human collagen {alpha}1(I)-like gene from a cosmid library. The clone which contains 37kb of human DNA has been shown to contain this gene by DNA sequencing, hybrid arrest and hybrid selection assays and Northern blot hybridizations. The collagen gene sequence extends through most of the cloned DNA and must, therefore, be at least 35kb in length.

+Present address: Nordisk Gentofte, Niels Steensenvej 6, 2820 Gentofte, Denmark


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[Abstract] [PDF]


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