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Nucleic Acids Research, 1982, Vol. 10, No. 6 2085-2092
© 1982

MOLECULAR BIOLOGY

Nucleotide sequence of an exceptionally long 5.8S ribosomal RNA from Crithidia fasciculata

Murray N. Schnare and Michael W Gray

Department of Biochemistry, Faculty of Medicine, Dalhousie University Halifax, Nova Scotia, Canada B3H 4H7

Received October 28, 1981. Revised February 1, 1982. Accepted February 12, 1982.

In Crithidia fasciculata, a trypanosomatid protozoan, the large ribosomal subunit contains five small RNA species (e, f, g, i, j) in addition to 5S rRNA [Gray, M.W. (1981) Mol. Cell, Biol. 1, 347–357]. The complete primary sequence of species i is shown here to be pAACGUGUmCGCGAUGGAUGACUUGGCUUCCUAUCUCGUUGA... AGAmACGCAGUSAAGUGCGAUAAGUGGUA{psi}CAAUUGmCAGAAUCAUUCAAUUACCGAAUCUUUGAACGCAAACGG... CGCAUGGGAGAAGCUCUUUUGAGUCAUCCCCGUGCAUGCCAUAUUCUCCAmGUGUCGAA(C)OH. This sequence establishes that species i is a 5.8S rRNA, despite its exceptional length (171–172 nucleotides). The extra nucleotides in C. fasciculata 5.8S rRNA are located in a region whose primary sequence and length are highly variable among 5.8S rRNAs, but which is capable of forming a stable hairpin loop structure (the "G+C-rich hairpin"). The sequence of C. fasciculata 5.8S rRNA is no more closely related to that of another protozoan, Acanthamoeba castellanii , than it is to representative 5,8S rRNA sequences from the other eukaryotic kingdoms, emphasizing the deep phylogenetic divisions that seem to exist within the Kingdom Protista.


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