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Nucleic Acids Research, 1982, Vol. 10, No. 7 2209-2223
© 1982

MOLECULAR BIOLOGY

Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells

Elisabetta Ullu and Marialuisa Melli

European Molecular Biology Laboratory Postfach 10.2209, 6900 Heidelberg, FRG

Received February 19, 1982. Accepted March 19, 1982.

We have cloned cDNA copies of in vitro adenylated 7S RNA of HeLa cells. The most representative clones in the library contain DNA fragments copied from the 7SL and 7SK small RNAs. The two classes of recombinants share no homology. The 7SL RNA contains at the 5' end of the molecule sequences homologous to the Alu sequence family. Hybridization to human genomic DNA shows that the 7SL and 7SK clones are homologous to two different families of repetitive sequences.


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