Skip Navigation

This Article
Right arrow Print PDF (3686K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Strauss, F.
Right arrow Articles by Prunell, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strauss, F.
Right arrow Articles by Prunell, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1982, Vol. 10, No. 7 2275-2293
© 1982

MOLECULAR BIOLOGY

Nucleosome spacing in rat liver cluomatin. A study with exonuclease III

François Strauss and Ariel Prunell

Institut de Recherche en Biologie Moléculaire, Centre National de la Recherche Scientifique, Université Paris 7, 2, Place Jussieu 75251 Paris Cedex 05, France

Received February 2, 1982. Accepted March 9, 1982.

Exonuclease III was used to uniformly trim DNA ends of micrococcal nuclease-prepared chromatin fragments down to the first major impediment encountered by the enzyme, which arises from the interaction of H1 with the nucleosome. This trimming, when performed on nucleosome dimers, allowed one to quantitatively determine the center-to-center distance of nucleosomes. This distance, of mean 198 base pairs, was found to essentially vary between about 180 and 215 base pairs, with extremes of 165 and 230 base pairs. Trimming of trimers further revealed that the overall arrangement of nucleosome center-to-center distances along the chromatin fiber is that expected on a statistical basis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Stein, Y. Dalal, and T. J. Fleury
Circle ligation of in vitro assembled chromatin indicates a highly flexible structure
Nucleic Acids Res., December 1, 2002; 30(23): 5103 - 5109.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Fragoso, W. D. Pennie, S. John, and G. L. Hager
The Position and Length of the Steroid-Dependent Hypersensitive Region in the Mouse Mammary Tumor Virus Long Terminal Repeat Are Invariant despite Multiple Nucleosome B Frames
Mol. Cell. Biol., June 1, 1998; 18(6): 3633 - 3644.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. R. Pehrson and J. R. Pehrson
Probing the Conformation of Nucleosome Linker DNA in Situ with Pyrimidine Dimer Formation
J. Biol. Chem., September 22, 1995; 270(38): 22440 - 22444.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.