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Nucleic Acids Research, 1982, Vol. 10, No. 7 2323-2335
© 1982

MOLECULAR BIOLOGY

Nucleosomes containing histones H1 or H5 are closely inteespersed in chromatin

Santiago Torres-Martinez1 and Adolfo Ruiz-Carrillo2

Max-Planck für Molekulare Genetik 1000 Berlin 33, Ihnestrasse 63-73, FRG

2To whom correspondence and reprint requests should be sent.

Received November 16, 1981. Revised February 22, 1982. Accepted March 4, 1982.

The distribution of histones H1 and H5 along chromatin fibers has been examined in the nucleated hen erythrocyte. Nucleosome oligomers, produced by micrococcal nuclease digestion of nuclei, were sequentially reacted with affinity-chromatography purified rabbit anti-H5 and sheep anti-rabbit antibodies. Quantitation of the relative amounts of H1 and H5 in the precipitated and supernatant fractions as a function of the oligomer number was consistent with a close interspersion of both types of histones, probably a random one. This conclusion was supported by the immunoprecipitation of longer chromatin fibers. This pattern of distribution appears to apply both to bulk chromatin and to chromatin inactivated during the maturation of the erythrocyte.

1Present address: Departamento de Genética, Universidad de Murcia, Murcia, Spain.

Present address: Biologie Moléculaire, Unité des Virus Oncogènes, Institut Pasteur, 25 rue du Dr. Roux, Paris 75015, Paris.


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J. Sun, R Wiaderkiewicz, and A Ruiz-Carrillo
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Science, July 7, 1989; 245(4913): 68 - 71.
[Abstract] [PDF]


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