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Nucleic Acids Research, 1982, Vol. 10, No. 7 2337-2351
© 1982

CHEMISTRY

A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates

G.A. van der Marel, C.A.A. van Boeckel, G. Wille and J.H. van Boom

Gorlaeus Laboratories, State University of Leiden P.O. Box 9502, 2300 RA Leiden, The Netherlands

Received March 4, 1982. Accepted March 22, 1982.

A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exemplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.


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