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Nucleic Acids Research, 1982, Vol. 10, No. 7 2419-2437
© 1982

MOLECULAR BIOLOGY

Carbodiimide modification analyse of aminoacylated yeast phenylalanine tRNA: evidence for change in the apex region

David C. Fritzinger+ and Maurille J. Fournier

Department of Biochemistry, University of Massachusetts Amherst, MA 01003, USA

Received January 12, 1982. Revised March 15, 1982. Accepted March 15, 1982.

The G- and U-specific reagent, carbodiimide was used to probe the solution structure of aminoacylated yeast phenylalanine tRNA. Both quantitative and qualitative changes in modification were observed when the modification patterns of tRNA-CCA(3'OH), tRNA-CCA(3'NH2) and phe-tRNA-CCA(3'NH2) were compared. Five nucleotides were modified in all cases, D16 and G20 in the D-loop, U33 and Gm34 in the anticodon loop and U47, in the region of the extra arm. Small changes occurred in the D-loop with incorporation of the adenosine analogue manifest as new, low levels of modification of G22 (D-stem) and a loss of sensitivity to Mg+2 in modification of D16. Aminoacylation resulted in new modification of G19, modification of a residue in the T{psi}CG sequence, and a 2.5-fold increase in modification of G22. Taken together the results show that sminoacylation causes increased exposure of bases in the apex region of the L-shaped molecule where the D- and {psi}-loops are joined. The effects observed could occur an a consequence of stable or dynamic changes in conformation.

+Present address: Department of Biochemistry, University of Bristol Medical School, Bristol BS8 ITD, U.K.


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