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Nucleic Acids Research, 1982, Vol. 10, No. 7 2439-2451
© 1982

ENZYMOLOGY

Induced hydrolytic activity of yeast phenylalanyl-tRNA synthetase by tRNAPhe-CC

W. Kuhn and F.W. Schneider

Institut für Physikalische Chemie der Universität Würzburg Marcusstr. 9/11, D-8700 Würzburg, FRG

Received January 5, 1982. Revised March 17, 1982. Accepted March 17, 1982.

"Induced hydrolysis" a new hydrolytic activity, was found by measuring AMP-production during aminoacylation of tRNAPhe-CCA by yeast phenylalanyl-tRNA synthetase in the presence of tRNAPhe-CC under conditions of low ionic strength at pH 8.5. Experiments using the elongation factor Tu·GTP provide evidence that transfer of phenylalanine to the tRNAPhe-CCA is followed by rapid hydrolysis in the presence of tRNAPhe-CC. A simple mechanism shows good agreement with the experimental data.


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