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Nucleic Acids Research, 1983, Vol. 11, No. 1 127-139
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of the ilvB multivalent attenuator region of Escherichia coli K12

Craig A. Hauser and G. Wesley Hatfield

Department of Microbiology, College of Medicine, University of California Irvine, CA 92717, USA

Received September 14, 1982. Revised December 1, 1982. Accepted December 1, 1982.

The ilvB gene of Escherichia coli K12 has been cloned into a multicopy plasmid. The regulation of the cloned gene by valine or leucine limitation and by catabolite repression is the same as for the chromosome encoded gene. The nucleotide sequence of a regulatory region preceding the ilvB structural gene has been determined. This DNA sequence includes a promoter, a region which codes for a putative 32 amino acid polypeptide containing multiple valine and leucine codons, and a transcription termination site. In vitro transcription of this region produces a 184 nucleotide terminated leader transcript. Mutually exclusive secondary structures of the leader transcript are predicted. On the basis of these data, a model for multivalent attenuation of the ilvB operon is presented. Data are presented which suggests that at least part of the postulated CRP-cyclic AMP binding site of the ilvB operon precedes the transcription start site by more than 71 base pairs.


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