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Nucleic Acids Research, 1983, Vol. 11, No. 10 2961-2977
© 1983

MOLECULAR BIOLOGY

The characterization of cDNA clones coding for wheat storage proteins

Dorothea Bartels and Richard D. Thompson

Cytogenetics Department, Plant Breeding Institute Maris Lane, Trumpington, Cambridge CB22LQ, UK

Received March 31, 1983. Accepted April 29, 1983.

Poly (A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the connstruction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode {alpha}/ß glidin related sequences and gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley stroge protein, B-hordein. The sequence includes a tendem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.


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