Skip Navigation

This Article
Right arrow Print PDF (4148K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (39)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Germond, J.-E.
Right arrow Articles by Wahli, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Germond, J.-E.
Right arrow Articles by Wahli, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 10 2979-2997
© 1983

MOLECULAR BIOLOGY

VlteBogtnin B2 gene in Xenopus laevis: Isolation, in vitro transcription and relation to other vitellogenin genes

Jacques-Edouard Germond*, Beatruce ten Heggeler*, Jean-Luc Schubiger*, Philippe Walker*, Bruce Westley+,1 and Walter Wahli*

*Institut de Biologie animale, Universiteède Lsausanee, Palais de Rurnine Ch-1005 Lausanne +Abteilung fur Zell-und Entwicklungsbiologie, Universitat Bern Baltzerstrasse 4, CH-3012

Received March 30, 1983. Accepted April 27, 1983.

The isolation of the four Xenopus laevis vitellogenin genes has been completed by the purification from a DNA library of the B2 gene together with its flanking sequences. The overlapping DNA fragments analyzed cover 34 kilobases. The B2 gene which has a length of 17.5 kilobases was charxacterized by heteroduplex and R-loop mapping 1n the electron microscope and by in vitro transcription 1n a HeLa whole-cell extract. Its structural organization is compared with that of the closely related Bl gene. The mRNA-coding sequence of about 6 kilobases 1s interrupted 34 times in the Bl gene and 33 times in the B2 gene. Sequence homology between the two genes was not only found in exons. In addition, 54% of the intron sequences as well as 6356 and 48.5% respectively of the 5' and 3' flanking sequences, show enough homology to form stable duplexes. These findings are compared with earlier results obtained with the two other closely related members of the vitellogenin gene family, the Al and the A2 genes

1Present address: Institute National de la santeè et de la Recherche Meèdicale (U 148), 60, Rue de Navacelles, F-34100 Montpellier, France.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
S. Sawaguchi, Y. Koya, N. Yoshizaki, N. Ohkubo, T. Andoh, N. Hiramatsu, C. V. Sullivan, A. Hara, and T. Matsubara
Multiple Vitellogenins (Vgs) in Mosquitofish (Gambusia affinis): Identification and Characterization of Three Functional Vg Genes and Their Circulating and Yolk Protein Products
Biol Reprod, April 1, 2005; 72(4): 1045 - 1060.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.