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Nucleic Acids Research, 1983, Vol. 11, No. 10 3027-3035
© 1983

MOLECULAR BIOLOGY

Transaltion of lymphocyte mRNA for mouse colony stimulating factor

R. C. Bleackley*, H. Horak+, J. McElhaney*, C. Havele*, A. R. E. Shaw+, A. R. Turner+ and V. H. Paetkau*

*Department of Biochemistry, University of Alberta, Edmonton Alberta, Canada T6G 2H7 +W. W.Cross Cancer Institute, Edmonton Alberta, Canada T6G 1Z2

Received March 23, 1983. Accepted April 20, 1983.

Poly A RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF) These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF


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J. Elliott, Y Lin, S. Mizel, R. Bleackley, D. Harnish, and V Paetkau
Induction of interleukin 2 messenger RNA inhibited by cyclosporin A
Science, December 21, 1984; 226(4681): 1439 - 1441.
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