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Nucleic Acids Research, 1983, Vol. 11, No. 10 3037-3045
© 1983

MOLECULAR BIOLOGY

The nucleotide sequence of Bacillus Subtuis tRNA genes

Yuko Yamada*, Misao Ohki+ and Hisayuki Ishikura*

*Laboratory of Chemistry, Jichi Medical School, Minarnikawachi-machi Tochigi-ken 329-04, Japan +Biology Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku Tokyo 104, Japan

Received March 18, 1983. Revised April 25, 1983. Accepted April 25, 1983.

Clones earring Bacillue aubtilis tRNA genes were isolated from a 003B3;816 library. A recombinant phage 003B3;816-BS83 which was hybridized effectively with unfractionated tRNA probes contained a 3-kb fragment. By a Southern's blot analysis, it was found that tRNA genes were located in Eco RI-Hinc II region of this fragment. Sequence determination revealed the presence of a cluster of four tRNA genes in this region. The gene organization was as follows: tDNALyS-9bp-tDNAGlU-81bp-tDNAAsP-30bp-tDNAPhe. The RNA sequences expected from tDNALys and tDNAphe were identical with the reported RNA sequences. Two tRNA genes, tDNALys and tDNAAsp encoded the CCA sequence of 3'-terminal region, but the other two, tDNAGlu and tDNAphe did not. A promoter-like sequence which corresponds to the 003C3;55-recognition site was found in a region about l00bp upstream from tDNALys


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