Skip Navigation

This Article
Right arrow Print PDF (2725K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Duggleby, C. J.
Right arrow Articles by Jones, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duggleby, C. J.
Right arrow Articles by Jones, S. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 10 3065-3076
© 1983

MOLECULAR BIOLOGY

Cloning and expression of the staphyiococcus aureus protein A gene in Escheirchia Coli

Clive J. Duggleby and Susan A. Jones

PHLS Centre for Applied Microbiology and Research Porton Down, Salisbury Wiltshire SP4 0JG UK

Received March 10, 1983. Revised April 15, 1983. Accepted April 15, 1983.

A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli Hb101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli was characterised in minicells and by Western blotting and double diffusion experiments


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Rheumatology (Oxford)Home page
S. P. Nair, R. J. Williams, and B. Henderson
Advances in our understanding of the bone and joint pathology caused by Staphylococcus aureus infection
Rheumatology, August 1, 2000; 39(8): 821 - 834.
[Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.