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Nucleic Acids Research, 1983, Vol. 11, No. 10 3137-3153
© 1983

MOLECULAR BIOLOGY

Unusual domains of human alphoid statllite DNA with contiguous non-satellite sequences: sequence analysis of a junction region

S. Steven Potter and Richard S. Jones

Biology Department, Wesleyan University Middletown, CT 06457, USA

Received February 18, 1983. Revised April 15, 1983. Accepted April 15, 1983.

The sequence organization of cloned segments of Human DNA carrying unusual domains of alphoid satellite was studied by restriction mapping, electron microscopy and base sequence analysis. In some cases restriction mapping revealed the absence of the typical 340 bp EcoR 1 diner, although blot hybridizations showed the extensive presence of alphoid satellite. A variant monomeric construction was demonstrated by DNA sequencing. Furthermore, inverted repeats within these domains were detected by electron microscopy. In one case these were shown to be the result of interruptions in the satellite sequence by members of a family of repetitive, conserved elements


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