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Nucleic Acids Research, 1983, Vol. 11, No. 10 3255-3267
© 1983

MOLECULAR BIOLOGY

Mouse Hepatic metallothionein-1 gene cleavage by micrococcal nuclease is enhanced after induction by cadmium

James Koropatnick*, Glen Andrews+, J.D. Duerksen*, Umesh Varshney and Lashitew Gedamu

*Department of Biology Calgary Alberta, Canada T2N 1N4 +Department of Medical Biochemistry, University of Calagary Calgary Alberta, Canada T2N 1N4 Division of Biochemistry and Department of Chemistry, University of Calgary Calgary, Alberta Canada, T2N 1N4

Received January 11, 1983. Revised April 28, 1983. Accepted April 28, 1983.

Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metalIoth1one1n (MT) gene 1n adult mouse liver, when induced to produce mfiNA by Injection of cadmium, becomes more susceptible to nuclease cleavage. However, the MT gene in uninduced liver, and the alphafoetal protein (AFP) gene in both induced and uninduced liver, remain relatively resistant to nuclease cleavage. The AFP gene is not normally expressed in cadmium induced or uninduced liver. Thus, susceptibility of genes to nuclease cleavage appears to rise with Increasing transcription of the gene


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