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Nucleic Acids Research, 1983, Vol. 11, No. 10 3351-3362
© 1983

MOLECULAR BIOLOGY

Cloning and sequence of UK bovine rotavirus gene segment 7: marked sequence homology with simban rotavirus gene segment 8

M. L Dyall-Smith*, T.C. Ellemant+, P.A. Hoyne+, I.H. Holmes* and A.A. Azad+

*University of Melbourne, Department of Micorobiolgy Gratta St., +CSIRO, Division of Proteing Chemiostry, Parkville Victoria 3052, Austrialia

Received March 25, 1983. Revised April 21, 1983. Accepted April 21, 1983.

The genome of the UK bovine rotavirus, which consists of eleven segments of dsRNA was polyadenylated and reverse-transcribed into cDNA. Complementary cDNA strands were annealed and the termini of the duplexes completed using DNA polymerase I. Full-length DNA copies of RNA segments 7,8 and 9 were cloned into the Pst I site of pBR322 and a clone containing the entire gene 7 was identified and sequenced. Gene 7 is 1059 nucleotides in length and contains a single long open reading frame capable of coding for a protein of 317 amino-acids. The known gene product of segment 7 is a protein with an estimated molecular weight of 33,000 daltons. When the UK bovine rotavirus gene 7 sequence was compared with the published data for the homologous gene (segment 8) of the simian rotavirus SA11, it was found to be identical to it in size and the arrangement of the proposed coding and non-coding regions, and very similar in nucleotide sequence (88% horaology). Most of the base changes are silent and the predicted amino-acid sequences are almost identical (96% homology)


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