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Nucleic Acids Research, 1983, Vol. 11, No. 11 3487-3502
© 1983

MOLECULAR BIOLOGY

Complex endonudeolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila

Klaus-Peter Kister, Brigitte Muller and Werner A. Eckert*

Department of Physiology, Institute of Zoology, University of Heidelberg D-6900 Heidelberg 1, FRG

*To whom correspondence should be sent:

Received April 15, 1983. Accepted May 16, 1983.

We have analysed nuclear RNA from T. thermophila by RNA transfer hybridization using cloned rDNA fragments. A very high number of in vivo intermediates and by-products of rRNA processing were identified. These Include putative intermediates of the splicing process and alternative products resulting from temporal variability in various endonucleolytic cleavages. In addition, four small RNA species including only transcribed spacer sequences were detected. These are (1) the IVS RNA ({small tilde}400 bases), the by-product of the splicing process, (2) a fragment from the internal transcribed spacer ({small tilde}36O bases), possibly resulting from 3’-end processing of pre-17S rRNA, (3) a fragment comprising most or all of the external transcribed spacer ({small tilde}600 bases) obviously representing the major by-product of 5’–-end processing, and, in addition, (4) a small fragment from the initiation region ({small tilde}230 bases) which might be a product of premature transcription termination.


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