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Nucleic Acids Research, 1983, Vol. 11, No. 11 3517-3530
© 1983

MOLECULAR BIOLOGY

Isolation of non-globin genes expressed preferentially in mouse erythroid cells

P. S. Goldfarb, J. O'Prey, N. Affara, Q.-S. Yang and P. R. Harrison

The Beatson Institute for Cancer Research, Garscube Estate Switchback Road, Bearsden, Glasgow G61 1BD, UK

Received April 13, 1983. Accepted May 16, 1983.

Genomic DNA recombinants were isolated from a library of Balb–C mouse genomic DNA fragments cloned in {lambda}Ch4A by screening with cDNA derived from 13d foetal liver cell or adult reticulocyte poly A+ RNA. Subsequent screening enabled us to identify non–globin genomic sequences whose expression appeared exclusive to or elevated in erythroid cells. Further analysis of the structure and expression of these sequences was performed using Southern blot and DNA or RNA dot hybridisation analysis. In one recombinant part of the cloned genomic sequence corresponded to an erythrcblast specific mRNA identified previously by Affara et al, (5).


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