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Nucleic Acids Research, 1983, Vol. 11, No. 11 3559-3569
© 1983

MOLECULAR BIOLOGY

The sequence GGCmCGG is resistant to MspI cleavage

M. Busslinger*, E. de Boer, S. Wright, F. G. Grosveld and R. A. Flavell+

Laboratory of Gene Structure and Expression NIMR, Mill Hill, London NW7 1AA, UK *Institut für Molekularbiologie II, University of Zürich Zürich, Switzerland +Biogen Research Corp. 241 Binney Street, Cambridge, MA 02142, USA

Received March 22, 1983. Accepted May 11, 1983.

MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.


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