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Nucleic Acids Research, 1983, Vol. 11, No. 11 3631-3636
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of a region downstream of the mouse CKimmunoglobulin gene

Peter S. Neumaier and Hans G. Zachau

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universitat München München, FRG

Received March 21, 1983. Revised May 17, 1983. Accepted May 17, 1983.

2318 bp downstream of the Ck (1) gene segment were sequenced in a clone (L1-D) derived from mouse liver DNA. The 966 bp at the 5’ side of this stretch were found to be identical to a sequence which had been determined previously in a myeloma T derived clone, i.e. no somatic mutations had occurred in the transition from the germline to the rearranged configuration. The remaining 13 52 bp had not been known and extend the sequenced part of the mouse Jk –Ck region to about 7.5 kb. Within the newly sequenced area three BspRI sites have been located which were used in chroraatin studies (Weischet et al., accompanying publications). In L1-D sequences have been found which are possible targets of aberrant recombination events.


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