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Nucleic Acids Research, 1983, Vol. 11, No. 12 3959-3971
© 1983

MOLECULAR BIOLOGY

Detection of non-homology-containing heteroduplex molecules

Michael J. Lichten and Maurice S. Fox

Massachusetts Institute of Technology, Department of Biology 77 Massachusetts Avenue, Cambridge, MA 02139, USA

Received April 7, 1983. Accepted May 9, 1983.

Heteroduplex DNA molecules which contain both the wild-type and mutant sequences of a deletion nonhomology possess a characteristic electrophoretic mobility in agarose and can be readily separated from both the wild-type and deletion-containing parental homoduplex fragments. Because of the partial single stranded character of these deletion-containing heteroduplex molecules, they are selectively bound to nitrocellulose filters, and once bound, can be selectively detected by hybridization with radioactively labeled single-stranded DNA which is homologous to the sequences absent in the deletion mutation.


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