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Nucleic Acids Research, 1983, Vol. 11, No. 12 3989-4006
© 1983

MOLECULAR BIOLOGY

Transcription of cloned Moloney murine leukemia proviral DNA injected into Xenopus laevis oocytes

Michael Breindl, Holger Kalthoff+ and Rudolf Jaenisch

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hambur, Institut für Physiologische Chemie, Universität Hamburg Martinistrasse 52, 2000 Hamburg 20, FRG +Abteilung Zellbiochemie, Institut für Physiologische Chemie, Universität Hamburg Martinistrasse 52, 2000 Hamburg 20, FRG

Received April 7, 1983. Revised May 24, 1983. Accepted May 24, 1983.

We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome copy of M-MuLV in their germ line at different chromosomal positions and differ from each other with respect to expression of the proviral genome. We show here that the different M-MuLV proviral genome copies were transcribed into virus-specific RNA with similar efficiencies. Transcription of viral RNA initiated correctly at the viral promoter in the 5' LTR, whereas the promoter in the 3' LTR was used with a much lower frequency, if at all, for initiation of RNA synthesis. Most of the virus-specific transcripts were smaller than authentic M-MuLV mRNA and confined to the oocyte nucleus. Using immunoprecipita-tion, we were not able to detect virus-specific proteins after injection of proviral DNA, whereas after injection of a comparable amount of M-MuLV mRNA viral protein was readily detectable.


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