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Nucleic Acids Research, 1983, Vol. 11, No. 12 4007-4017
© 1983

MOLECULAR BIOLOGY

Immunoglobulin diversity: analysis of the germ-line VH gene repertoire of the murine anti-GAT response

Claudine Schiff, Michelé Milili and Michel Fougereau

Centre d'Immunologie INSERM-CNRS de Marseille-Luminy Case 906 13288 Marseille Cedex 9, France

To whom reprint requests should be sent

Received March 29, 1983. Revised May 27, 1983. Accepted May 27, 1983.

A cDNA clone was constructed from a mRNA encoding an anti-GAT (Glu60 Ala30 Tyr10 ) BALB/c monoclonal antibody heavy chain. Its sequence, covering codons –5 to 162 and therefore encompassing the complete V-D-J region, was determined. Surprisingly, the sequence of the VH gene-encoded region was almost identical with that of the BALB/c VH anti-NP (4-hydroxy-3-nitrophenyl) acetyl VH region, suggesting that the same VH germ-line might be used to encode two heavy chains contributing to antibodies of discrete specificities. A specific VH probe was derived and annealed to Eco RI and bgl II restriction fragments of liver (unrearranged) DNA extracted from the BALB/c, DBA/2 and C57BL/6 mouse strains that differ in their H chain allotypes. Under stringent conditions, only a few bands were identified by Southern blotting. The different patterns observed suggest that the VH anti-GAT repertoire differs between these strains eventhough their various anti-GAT antibodies express the same public idiotypic specificities.


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