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Nucleic Acids Research, 1983, Vol. 11, No. 12 4177-4183
© 1983


MOLECULAR BIOLOGY

A number of different nuclear genes for the small subunit of RuBPCase are transcribed in petunia

Pamela Dunsmuir1, Steven Smith2 and John Bedbrook3

CSIRO Division of Plant Industry P.O.Box 1600, Canberra City, A.C.T. 2601, Australia

1To whom correspondence should be addressed

Received January 17, 1983. Revised May 18, 1983. Accepted May 18, 1983.

The sequences in the petunia genome which encode the small subunit polypeptides of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase have been characterized. Sequence analysis of four cDNA clones indicates that there are several distinct genes transcribed in leaf tissue. There is 8–9% nucleotide divergence between the transcripts however these changes do not alter the encoded amino acid sequence. Examination of nuclear DNA by Southern hybridization and analysis of cloned small subunit genes confirm that there are a number of different genes which encode this single protein.


2Current address: Department of Botany, University of Edinburgh, Edinburgh EH6 3JH, UK

3Current address: Advanced Genetic Sciences Inc., P.O.Box 3266, Berkeley, CA 94703, USA


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