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Nucleic Acids Research, 1983, Vol. 11, No. 12 4185-4193
© 1983

MOLECULAR BIOLOGY

The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and {alpha}

Janet A. Hall* and Roy Saffhill

Paterson Laboratories, Christie Hospital and Holt Radium Institute Manchester M20 9BX, UK

Received April 21, 1983. Revised May 27, 1983. Accepted May 27, 1983.

The modified nucleoside 5'-triphosphates 06-MedGTP and 04-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and {alpha} in an in vitro assay. 06-MedGMP is only incorporated into newly-synthesised DNA-like material in the presence of templates containing thymine bases. Similarly 04-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of 06-MeG and 04-MeT.

*Present address: International Agency for Research on Cancer, 150 Cours Albert Thomas, Lyon, France


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