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Nucleic Acids Research, 1983, Vol. 11, No. 12 4251-4256
© 1983

MOLECULAR BIOLOGY

Blocked 5'-termini in the fragments of chromosomal DNA produced in cells exposed to the antitumour drug 4'-[(9-acridinyl)-amino]methanesulphon-m-anisidide (mAMSA)

B. Marshall1, R.K. Ralph2 and R. Hancock2

1Cell Biology Department, University of Auckland Auckland, New Zealand 2Swiss Institute for Experimental Cancer Research 1066 Epalinges/Lausanne, Switzerland

*To whom correspondence should be sent

Received March 14, 1983. Revised May 20, 1983. Accepted May 20, 1983.

Comparison of the sensitivity of DNA isolated from untreated and mAMSA-treated PY815 mouse mastocytoma cells to hydrolysis by E.coli 3'-exonuclease III and phage {lambda} or phage T7 5'-exonuc1eases show that the fragments of chromosomal DNA produced by mAMSA treatment have free 3'-OH termini and blocked 5'-termini.


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