Skip Navigation

This Article
Right arrow Print PDF (849K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (65)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vedel, M.
Right arrow Articles by Sala-Trepat, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vedel, M.
Right arrow Articles by Sala-Trepat, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 13 4335-4354
© 1983

MOLECULAR BIOLOGY

Changes in methylation pattern of albumin and {alpha}-fetoprotein genes in developing rat liver and neoplasia

Michèle Vedel1,, Micaela Gomez-Garcia2,, Maria Sala* and Josè M. Sala-Trepat+,

*Institut de Recherches Scientifiques sur le Cancer, CNRS 94800 Villejuif, France Laboratoire d'Enzymologie du CNRS 91190 Gif-sur-Yvette

+To whom correspondence should be sent

Received April 28, 1983. Revised June 3, 1983. Accepted June 3, 1983.

To determine whether methylation changes in specific DNA sequences of the albumin and AFP genes are implicated in the modulation of transcriptional activity during rat liver development and neoplasia we have analysed the methylation pattern of C-C-G-G sequences within these genes in DNA isolated from fetal and adult hepatocytes, from adult kidney and from a clonal hepatoma cell line which produces AFP but no albumin. We have assayed for methylation of the internal cytosine of this sequence by using the restriction enzyme isoschizomers HpaII and MspI. 32P-labelled cloned cDNA probes were used to reveal the albumin and AFP gene containing fragments. Genomic subclones of the albumin gene were also utilized as molecular probes to measure quantitatively the level of methylation of 6 specific sites within the albumin gene in the different DNA samples. The results indicate that methylation changes at the sites analysed are not responsible for the changes in gene activity during rat liver development. Further they demonstrate that : 1) extensively methylated genes can be actively transcribed ; 2) prominent changes in methylation of specific genes during normal development are not necessarily related to alterations in gene activity.

1Permanent address: Institut de Chimie des Substances Naturelles, CNRS, 91190 Gif-sur-Yvette, France

2On leave of absence from Departamento de Bioquimica, Facultad de Farmacia, Valencia, Spain


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
H. Trompeter, A Schiermeyer, G Blankenburg, E Hennig, and H. Soling
Factors involved in the cell density-dependent regulation of nuclear/cytoplasmic distribution of the 11.5-kDa Zn(2+)-binding protein (parathymosin-alpha) in rat hepatocytes
J. Cell Sci., January 11, 1999; 112(22): 4113 - 4122.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.