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Nucleic Acids Research, 1983, Vol. 11, No. 13 4409-4415
© 1983


ENZYMOLOGY

A new restriction endonuclease from Acelobacter pasteurianus

J. Seurinck1,*, A. Van de Voorde2 and M. Van Montagu1,+

1Laboratorium voor Genetica, Rijksuniversiteit Gent B-9000 Gent, Belgium 2Laboratorium voor Moleculaire Biologie Rijksuniversiteit Gent, B-9000 Gent, Belgium

+To whom correspondence should be sent

Received March 28, 1983. Revised June 1, 1983. Accepted June 8, 1983.

A restriction endonuclease, Apal, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage {lambda} DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave {pi}x174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence Formula and cuts at the sites indicated by the arrows.


*Present address: Plant Genetic Systems, Plateaustraat 22, B-9000 Gent, Belgium


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