Nucleic Acids Research, 1983, Vol. 11, No. 13 4409-4415
© 1983
ENZYMOLOGY |
A new restriction endonuclease from Acelobacter pasteurianus
1Laboratorium voor Genetica, Rijksuniversiteit Gent B-9000 Gent, Belgium 2Laboratorium voor Moleculaire Biologie Rijksuniversiteit Gent, B-9000 Gent, Belgium
+To whom correspondence should be sent
Received March 28, 1983. Revised June 1, 1983. Accepted June 8, 1983.
A restriction endonuclease, Apal, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage
DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave
x174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence
and cuts at the sites indicated by the arrows.
*Present address: Plant Genetic Systems, Plateaustraat 22, B-9000 Gent, Belgium